ABSTRACT
Abstract Objective To evaluate the antitumoral role of γδ TDC cells and αβ TDC cells in an experimental model of breast cancer. Methods Thirty female Balb/c mice were divided into 2 groups: control group (n=15) and induced-4T1 group (n=15), in which the mice received 2 x 105 4T1 mammary tumor cell line. Following the 28-day experimental period, immune cells were collected from the spleen and analyzed by flow cytometry for comparison of αβ TDC (TCRαβ+ CD11c+MHCII+) and γδ TDC (TCRγδ+CD11c+MHCII+) cells regarding surface markers (CD4+ and C8+) and cytokines (IFN-γ, TNF-α, IL-12 and IL-17). Results A total of 26.53% of γδ TDC- control group (p<0.0001) - the proportion of αβ TDC was lower in splenic cells than γδ TDC; however, these 2 cell types were reduced in tumor conditions (p<0.0001), and the proportion of IFN-γ, TNF-α, IL-12 and IL-17 cytokines produced by γδ TDC was higher than those produced by αβ TDC, but it decreased under conditions of tumor-related immune system response (p<0.0001). Conclusion Healthy mice engrafted with malignant cells 4T1 breast tumor presented TDC with γδ TCR repertoire. These cells express cytotoxic molecules of lymphocytes T, producing anti-tumor proinflammatory cytokines.
Resumo Objetivo Esclarecer o possível papel antitumoral das células TDC γδ e TDC αβ em um modelo experimental de câncer de mama. Métodos Trinta baços de camundongos Balb/c analisados por citometria de fluxo, separados entre grupo controle (n=15) e o grupo tumoral induzido por 4T1 (n=15). Resultados Presença de 26,53% de TDC γδ nos camundongos do grupo controle (p<0,0001), proporção de TDC αβ menor em células esplênicas do que TDC γδ; no entanto, estes dois tipos de células são reduzidos emcondições tumorais (p<0,0001), e a proporção de citocinas IFN-γ, TNF-α, IL-12 e IL-17 produzidas pelas célula TDC γδ foi maior do que as produzidas pelas células TDC αβ, mas foram diminuídas sob condições de resposta ao sistema imunológico relacionada ao tumor (p<0,0001). Conclusão Camundongos saudáveis induzidos ao tumor de mama 4T1 apresentaram TDC com repertório TCR γδ. Estas células expressam moléculas citotóxicas de linfócitos T, produzindo citocinas proinflamatórias anti-tumor.
Subject(s)
Animals , Female , Mice , Breast Neoplasms/immunology , Receptors, Antigen, T-Cell, gamma-delta/immunology , Receptors, Antigen, T-Cell, gamma-delta/metabolism , Receptors, Antigen, T-Cell, alpha-beta/immunology , Receptors, Antigen, T-Cell, alpha-beta/metabolism , Spleen/immunology , Spleen/metabolism , Interleukin-17 , Flow Cytometry , Mice, Inbred BALB CABSTRACT
Este trabalho objetiva caracterizar a organização, a articulação e a assistência da rede de atenção à saúde no estado da Paraíba, com base nos dados do 1º ciclo de Avaliação Externa do Programa Nacional de Melhoria do Acesso e da Qualidade da Atenção Básica (PMAQ-AB). Realizou-se um estudo do tipo transversal, cujos dados foram analisados descritivamente por meio de valores absolutos e respectivos percentuais. Concluiu-se que existem fragilidades na integração da rede de atenção à saúde na Paraíba em relação ao ordenamento e definição do fluxo, em especial na contrarreferência, fato que pode comprometer a integralidade e o papel da Atenção Básica de coordenar o cuidado e ordenar as redes.
In this work, are analyzed the organization, coordination and assistance of the health care networks in the state of Paraíba, Brazil, based on the data obtained from the 1st cycle of the external evaluation of the National Program for Access and Quality Improvement in Primary Care (PMAQ-AB). It was carried on a cross-sectional study and the data were descriptively analyzed by its absolute and percentage values. It was concluded that there are weaknesses in the integration of the health care network in the state of Paraíba/Brazil concerning the planning and definition of the flow, especially in counter-reference which may compromise the integrity and the role of primary care in order to manage public care and health network.